COLICINE V

Colicine-susceptibility patterns of enteropathogenic Escherichia coli.

The Isolation of Colicine V and a Study of Its Immunological Properties

Colicine V has been obtained from the culture medium in which the colicinogenic bacillus E. coli K357 L_T is grown. The material is electrophoretically homogeneous and proves to be a lipocarbohydrate protein complex identical with the type-specific O antigen of the parent bacillus. Colicine V is toxic both for mice and for rabbits and readily stimulates the elaboration of precipitins and bacter...

Comparison of colicine production and diffusion on different solid media.

Using a freezing-thawing method for extracting colicine from solid media it has been shown that the choice of media for production and diffusion is important. Digest nutrient agar yielded the most colicine and peptone water agar the least. A factor in bacteriological peptone, but absent in a proteose peptone (Difco) and Neopeptone, was responsible for inhibiting production on peptone water agar...

Enhancement of Antibiotic Activity of Colicine K and Alteration of Serological Properties of Colicine K and Endotoxins

Hemoglobin, its chains, and myoglobin enhance the antibiotic activity of colicine K. These proteins also interact with colicine K and other O antigens to alter their serological activity. The hemoglobin proteins did not alter the serological activities of three Pneumococcus polysaccharides or T4 bacteriophage DNA antigens but did alter the antigenic activity of fetuin. Interaction of hemoglobin...

COLICINE K VII. THE TRANS~V.R OF TYPE K COLICmOGENY

There can be but little doubt that the bactericidal activity of the colieine K which is elaborated by F~cherichia coli K235 resides in the protein portion of the bacterial somatic antigen (1). In its native state this component is firmly bound to a lipopolysaccharide and the two cannot be separated by the usual chemical or physical techniques. The question has arisen, however, as to what might ...